203 research outputs found
Estimating Spectroscopic Redshifts by Using k Nearest Neighbors Regression I. Description of Method and Analysis
Context: In astronomy, new approaches to process and analyze the
exponentially increasing amount of data are inevitable. While classical
approaches (e.g. template fitting) are fine for objects of well-known classes,
alternative techniques have to be developed to determine those that do not fit.
Therefore a classification scheme should be based on individual properties
instead of fitting to a global model and therefore loose valuable information.
An important issue when dealing with large data sets is the outlier detection
which at the moment is often treated problem-orientated. Aims: In this paper we
present a method to statistically estimate the redshift z based on a similarity
approach. This allows us to determine redshifts in spectra in emission as well
as in absorption without using any predefined model. Additionally we show how
an estimate of the redshift based on single features is possible. As a
consequence we are e.g. able to filter objects which show multiple redshift
components. We propose to apply this general method to all similar problems in
order to identify objects where traditional approaches fail. Methods: The
redshift estimation is performed by comparing predefined regions in the spectra
and applying a k nearest neighbor regression model for every predefined
emission and absorption region, individually. Results: We estimated a redshift
for more than 50% of the analyzed 16,000 spectra of our reference and test
sample. The redshift estimate yields a precision for every individually tested
feature that is comparable with the overall precision of the redshifts of SDSS.
In 14 spectra we find a significant shift between emission and absorption or
emission and emission lines. The results show already the immense power of this
simple machine learning approach for investigating huge databases such as the
SDSS.Comment: accepted for publication in A&
Properties of optically selected BL Lac candidates from the SDSS
\textbf{Context.} Deep optical surveys open the avenue for find large numbers
of BL Lac objects that are hard to identify because they lack the unique
properties classifying them as such. While radio or X-ray surveys typically
reveal dozens of sources, recent compilations based on optical criteria alone
have increased the number of BL Lac candidates considerably. However, these
compilations are subject to biases and may contain a substantial number of
contaminating sources. \textbf{Aims.} In this paper we extend our analysis of
182 optically selected BL Lac object candidates from the SDSS with respect to
an earlier study. The main goal is to determine the number of bona fide BL Lac
objects in this sample. \textbf{Methods.} We examine their variability
characteristics, determine their broad-band radio-UV SEDs, and search for the
presence of a host galaxy. In addition we present new optical spectra for 27
targets with improved S/N with respect to the SDSS spectra. \textbf{Results.}
At least 59% of our targets have shown variability between SDSS DR2 and our
observations by more than 0.1-0.27 mag de- pending on the telescope used. A
host galaxy was detected in 36% of our targets. The host galaxy type and
luminosities are consistent with earlier studies of BL Lac host galaxies.
Simple fits to broad-band SEDS for 104 targets of our sample derived
synchrotron peak frequencies between with a peak at
. Our new optical spectra do not reveal any new
redshift for any of our objects. Thus the sample contains a large number of
bona fide BL Lac objects and seems to contain a substantial fraction of
intermediate-frequency peaked BL Lacs.Comment: Accepted for publication in A\&
Bose-Einstein condensation in a circular waveguide
We have produced Bose-Einstein condensates in a ring-shaped magnetic
waveguide. The few-millimeter diameter non-zero bias ring is formed from a
time-averaged quadrupole ring. Condensates which propagate around the ring make
several revolutions within the time it takes for them to expand to fill the
ring. The ring shape is ideally suited for studies of vorticity in a
multiply-connected geometry and is promising as a rotation sensor.Comment: 4 pages, 4 figure
Notions and subnotions in information structure
Three dimensions can be distinguished in a cross-linguistic account of information structure. First, there is the definition of the focus constituent, the part of the linguistic expression which is subject to some focus meaning. Second and third, there are the focus meanings and the array of structural devices that encode them. In a given language, the expression of focus is facilitated as well as constrained by the grammar within which the focus devices operate. The prevalence of focus ambiguity, the structural inability to make focus distinctions, will thus vary across languages, and within a language, across focus meanings
CD20 and CD19 targeted vectors induce minimal activation of resting B lymphocytes
B lymphocytes are an important cell population of the immune system. However, until recently it was not possible to transduce resting B lymphocytes with retro- or lentiviral vectors, making them unsusceptible for genetic manipulations by these vectors. Lately, we demonstrated that lentiviral vectors pseudotyped with modified measles virus (MV) glycoproteins hemagglutinin, responsible for receptor recognition, and fusion protein were able to overcome this transduction block. They use either the natural MV receptors, CD46 and signaling lymphocyte activation molecule (SLAM), for cell entry (MV-LV) or the vector particles were further modified to selectively enter via the CD20 molecule, which is exclusively expressed on B lymphocytes (CD20-LV). It has been shown previously that transduction by MV-LV does not induce B lymphocyte activation. However, if this is also true for CD20-LV is still unknown. Here, we generated a vector specific for another B lymphocyte marker, CD19, and compared its ability to transduce resting B lymphocytes with CD20-LV. The vector (CD19ds-LV) was able to stably transduce unstimulated B lymphocytes, albeit with a reduced efficiency of about 10% compared to CD20-LV, which transduced about 30% of the cells. Since CD20 as well as CD19 are closely linked to the B lymphocyte activation pathway, we investigated if engagement of CD20 or CD19 molecules by the vector particles induces activating stimuli in resting B lymphocytes. Although, activation of B lymphocytes often involves calcium influx, we did not detect elevated calcium levels. However, the activation marker CD71 was substantially up-regulated upon CD20-LV transduction and most importantly, B lymphocytes transduced with CD20-LV or CD19ds-LV entered the G1b phase of cell cycle, whereas untransduced or MV-LV transduced B lymphocytes remained in G0. Hence, CD20 and CD19 targeting vectors induce activating stimuli in resting B lymphocytes, which most likely renders them susceptible for lentiviral vector transduction
Magnetic trapping of ultracold neutrons
Three-dimensional magnetic confinement of neutrons is reported. Neutrons are
loaded into an Ioffe-type superconducting magnetic trap through inelastic
scattering of cold neutrons with 4He. Scattered neutrons with sufficiently low
energy and in the appropriate spin state are confined by the magnetic field
until they decay. The electron resulting from neutron decay produces
scintillations in the liquid helium bath that results in a pulse of extreme
ultraviolet light. This light is frequency downconverted to the visible and
detected. Results are presented in which 500 +/- 155 neutrons are magnetically
trapped in each loading cycle, consistent with theoretical predictions. The
lifetime of the observed signal, 660 s +290/-170 s, is consistent with the
neutron beta-decay lifetime.Comment: 17 pages, 18 figures, accepted for publication in Physical Review
Imaging cytoplasmic cAMP in mouse brainstem neurons
<p>Abstract</p> <p>Background</p> <p>cAMP is an ubiquitous second messenger mediating various neuronal functions, often as a consequence of increased intracellular Ca<sup>2+ </sup>levels. While imaging of calcium is commonly used in neuroscience applications, probing for cAMP levels has not yet been performed in living vertebrate neuronal tissue before.</p> <p>Results</p> <p>Using a strictly neuron-restricted promoter we virally transduced neurons in the organotypic brainstem slices which contained pre-Bötzinger complex, constituting the rhythm-generating part of the respiratory network. Fluorescent cAMP sensor Epac1-camps was expressed both in neuronal cell bodies and neurites, allowing us to measure intracellular distribution of cAMP, its absolute levels and time-dependent changes in response to physiological stimuli. We recorded [cAMP]<sub>i </sub>changes in the micromolar range after modulation of adenylate cyclase, inhibition of phosphodiesterase and activation of G-protein-coupled metabotropic receptors. [cAMP]<sub>i </sub>levels increased after membrane depolarisation and release of Ca<sup>2+ </sup>from internal stores. The effects developed slowly and reached their maximum after transient [Ca<sup>2+</sup>]<sub>i </sub>elevations subsided. Ca<sup>2+</sup>-dependent [cAMP]<sub>i </sub>transients were suppressed after blockade of adenylate cyclase with 0.1 mM adenylate cyclase inhibitor 2'5'-dideoxyadenosine and potentiated after inhibiting phosphodiesterase with isobutylmethylxanthine and rolipram. During paired stimulations, the second depolarisation and Ca<sup>2+ </sup>release evoked bigger cAMP responses. These effects were abolished after inhibition of protein kinase A with H-89 pointing to the important role of phosphorylation of calcium channels in the potentiation of [cAMP]<sub>i </sub>transients.</p> <p>Conclusion</p> <p>We constructed and characterized a neuron-specific cAMP probe based on Epac1-camps. Using viral gene transfer we showed its efficient expression in organotypic brainstem preparations. Strong fluorescence, resistance to photobleaching and possibility of direct estimation of [cAMP] levels using dual wavelength measurements make the probe useful in studies of neurons and the mechanisms of their plasticity. Epac1-camps was applied to examine the crosstalk between Ca<sup>2+ </sup>and cAMP signalling and revealed a synergism of actions of these two second messengers.</p
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